BIO 407 - Molecular Biology

1)  Inventory of research process:

• Library - Smart Search
• Library - Subject Databases... which ones?
• Google
• Google Scholar
• Mendeley

Pros (What worked) / Cons (What did not work so well): 

2) Research Process:

• Research Cycle > Student Scholarship Center Guide

3) Search Strategy:

• Keywords
• Subject Terms
• Focus: Species
• Focus: Gene (ie.  APPL)
• Focus: Gene expression or gene function or gene targeting or genetic regulation or genetic transcription or functional analysis or cell differentiation
• Focus: Disease or (brain OR neuro* OR neural*) or evolution

PICO (Population, Intervention, Comparison, Outcome)

Pros (What worked) / Cons (What did not work so well):

4) Critical Analysis of Results:

• 5-W's (Who, What, When, Where, Why)
• CRAP Test (Currency, Reliablility, Authority, Purpose or POV)
• Focus on Research Question

Pros (What worked) / Cons (What did not work so well):

D. ananassae

D. erecta

D. grimshawi

D. melanogaster

D. mojavensis

D. persimilis

D. pseudoobscura

D. sechellia

D. simulans

D. virilis

D. willistoni

D. yakuba

Minute Paper

1) Name

2) Species

3) Topic

4) What do you want to know to make your research easier?

Research Process Discussion:

1)  Search the following terms in Smart Search &  Medline

Alzheimers

Gene expression

Drosopila

2) Search the terms in Medline MESH index

3) Compare results

Article: Udai Bhan Pandey and Charles D. Nichols. (2011) Human Disease Models in Drosophila melanogaster and the Role of the Fly in Therapeutic Drug Discovery. Pharmacological Reviews June 2011 vol. 63 no. 2 411-436. doi: 10.1124/pr.110.003293   

Disease: Alzheimer's

Gene: APPL (APP in humans)

The following articles about Alzheimers are from the Reference List.  Using the Online Journal List, locate the article.

1) Crowther DC, Kinghorn KJ, Miranda E, Page R, Curry JA, Duthie FA, Gubb DC, Lomas DA (2005) Intraneuronal Abeta, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer's disease. Neuroscience 132:123–135.

2) Drake J, Link CD, Butterfield DA.  (2003) Oxidative stress precedes fibrillar deposition of Alzheimer's disease amyloid beta-peptide (1–42) in a transgenic Caenorhabditis elegans model. Neurobiol Aging 24:415–520.

3) Finelli A, Kelkar A, Song HJ, Yang H, Konsolaki M. (2004) A model for studying Alzheimer's Abeta42-induced toxicity in Drosophila melanogaster. Mol Cell Neurosci 26:365–375.

4) Hassan WM, Merin DA,Fonte V, Link CD (2009) AIP-1 ameliorates beta-amyloid peptide toxicity in a Caenorhabditis elegans Alzheimer's disease model. Hum Mol Genet 18:2739–2747.